A simple and reliable method for distinguishing danshen in salvia: simultaneous quantification of six active compositions by HPLC.
نویسندگان
چکیده
A simple and reliable method for distinguishing Danshen is important to evaluate the quality and clinical efficiency of these species. An HPLC method was developed for the determination of protocatechuic aldehyde, salvianolic acid A, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA in 23 samples of Salvia. The analytes were separated on an Agilent XDB C18 reversed-phase column coupled with a Phenomenex C18 guard column using a gradient elution of acetonitile-0.1% aqueous phosphoric acid as the mobile phase at a flow rate 0.8 mL/min and UV detection at 280 nm. The method allowing the simultaneous quantification of six major active compositions was optimized and validated for linearity, precision, accuracy and limits of detection (LOD) and quantification. The LOD ranged from 0.019 to 0.850 µg/mL (R(2) ≥ 0.9998). Accuracy, precision and reproducibility were all within the required limits. The average recovery between 96.49 and 102.16% and the relative standard deviations were <3.01%. Based on the six compositions content and clustering result, this research results suggest that these six major active compositions could be distinguishing markers for Danshen and non-Danshen.
منابع مشابه
Simultaneous Determination of Six Benzodiazepines in Spiked Soft Drinks by High Performance Liquid Chromatography with Ultra Violet Detection (HPLC-UV)
A high performance liquid chromatographic method with ultra violet detection for simultaneous analysis of six benzodiazepines (BZDs) (chlordiazepoxide, diazepam, clonazepam, midazolam , flurazpam and lorazepam) has been developed for forensic screening of adulterated non-alcoholic drinks. Samples were analyzed after a simple procedure for preparation using pH adjustment and filtering. Isocratic...
متن کاملSimultaneous determination of six phenolic constituents of danshen injection in rat plasma by liquid chromatography–electrospray ionization–mass spectrometry and its application to a pharmacokinetic study
Danshen, the root of Salvia miltiorrhiza, is one of the most commonly used traditional chinese herbs. owing to its remarkable and reliable biological activities, especially in the treatment of cardiovascular disorders, it is widely used either alone or in combination with other herbal ingredients for patients with coronary artery disease in china and other countries.1 Phenolic acids and lipophi...
متن کاملSimultaneous Determination of Six Benzodiazepines in Spiked Soft Drinks by High Performance Liquid Chromatography with Ultra Violet Detection (HPLC-UV)
A high performance liquid chromatographic method with ultra violet detection for simultaneous analysis of six benzodiazepines (BZDs) (chlordiazepoxide, diazepam, clonazepam, midazolam , flurazpam and lorazepam) has been developed for forensic screening of adulterated non-alcoholic drinks. Samples were analyzed after a simple procedure for preparation using pH adjustment and filtering. Isocratic...
متن کاملSimultaneous Determination of Carbazoles in Water Samples by Cloud Point Extraction Coupled to HPLC
Cloud point extraction (CPE) as a rapid, simple and efficient method coupled with high performance liquid chromatography (HPLC) was used for sample preparation and subsequent determination of carbazole, trinitrocarbazole (TrNC) and tetra nitro carbazole (TNC) in water samples. Some effective parameters on extraction, such as volume of Triton X-100, extraction time, extraction temperature, ionic...
متن کاملImproved quality control method for Fufang Danshen preparations through simultaneous determination of phenolic acids, saponins and diterpenoid quinones by HPLC coupled with diode array and evaporative light scattering detectors.
A herb-combined prescription, mainly derived from roots of Salvia miltiorrhiza and Panax notoginseng, has been widely used for improving coronary or cerebral circulation in China as well as in Western countries. Multiple commercially available preparations, known as Fufang Danshen preparations (FDPs), produced by various manufacturers with the raw materials from different sources, pose a seriou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of chromatographic science
دوره 52 9 شماره
صفحات -
تاریخ انتشار 2014